She has ruled out running in the upcoming Tasmanian election, but left open the option of another tilt at federal politics, saying she may consider running in the seat of Braddon if Labor MP Justine Keay is forced to resign over her citizenship.
Jacqui Lambie, whose father was born in Scotland, told colleagues she will leave Parliament.
The Tasmanian independent senator Jacqui Lambie has confirmed she will resign from parliament because she is a dual citizen but she has vowed to press on with her political career.
The senator told Tasmania Talks radio on Tuesday morning that she would resign after discovering that her father had not renounced his United Kingdom citizenship and it was "quite clear" that it meant she was also Scottish.
'If I am a dual citizen I will resign.
In Canberra, Senator Lambie attended Question Time in the Senate, but her office has not responded to questions from the media.
A High Court hearing into the matter found the deputy prime minister, Barnaby Joyce, was elected in breach of the Constitution.
Government backbencher John Alexander resigned on the weekend because he is a dual UK-Australian citizen, but he'll contest a by-election for his old seat of Bennelong on December 16.
While Senator Lambie said last week she was confident there was no issue with her citizenship, she is now preparing to quit parliament. Are you sad to see Lambie go?
"I'm happy to put on record that I'm satisfied that my parents are both Australian citizens and I have no concerns about me being a dual citizen because of where they were born or came from, in the case of my father, as an infant", she said in a media statement.
She is now the second Tasmanian senator to force a recount and replacement process, after the departure of Parry.
According to the constitutional expert, the High Court would need to decide if a local council position is an "office of profit under the Crown".
Lambie, who first entered the senate in 2013 as a member of Clive Palmer's Palmer United Party, left, and was re-elected as an independent senator a year ago.
Rural Health Tasmania's annual report for 2017 said it received funding from several federal government programs run by the departments of health and social services.