The sprint legend, who had won the 100m, 200m and 4x100m Gold at the same venue during the 2012 Olympics, might not have had a flawless end to his career, but his records over the years will definitely make him a once-in-a-lifetime athlete.
But after tonight's triumph, he said: "2012 didn't go our way and we have been working hard since then".
Gatlin smiled as he replied with the quip: "Yeah, I've been around a long time".
Jamaica were afforded a rousing welcome from the crowd, a relaxed-looking Bolt applauding the stands, with pictures of him constantly shown on the stadium's big screens.
Farah, who won the 10,000 title in the opening day at the London Stadium, settled for silver behind Ethiopian Muktar Edris in his final championship race on the track.
But the championships will be remembered for an anti-climactic end to the career of Bolt, a 19-time global champion who is arguably the greatest sprinter the world has ever seen.
Hosts Great Britain took the gold medal with a time of 37.47 seconds, and Usain Bolt's Jamaica team ended up not placing after his injury.
Jamaican team doctor Dr Kevin Jones later confirmed that Bolt had suffered from "cramp in his left hamstring".
"We've got to contend with the best athletes in the world".
They revealed they were made wait for 40 minutes in a cold call room ahead of the race.
Bolt was his long-time rival and he lost five successive Olympic and World Championship races to him.
Justin Gatlin, who snatched a gold in 100m dash to spoil Bolt's individual farewell race, suggested the same. The fans, however, are looking forward to seeing their superstar in action one last time as he takes to the track.
While Usain Bolt was cheered as he received his bronze medal but Gatlin got the opposite reaction.
"A hero of the sport to go down like that, as a true friend, I didn't like it one bit", Blake said.
"This is the best feeling in the world, world champions", said Gemili, overlooked for individual selection for the championships.